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A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast

The technologies for chromosome modification developed to date are not satisfactorily universal, owing to the typical requirements for special enzymes and sequences. In the present report, we propose a new approach for chromosome modification in Schizosaccharomyces pombe that does not involve any sp...

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Detalhes bibliográficos
Main Authors: Hirashima, Kyotaro, Iwaki, Tomoko, Takegawa, Kaoru, Giga-Hama, Yuko, Tohda, Hideki
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1351375/
https://ncbi.nlm.nih.gov/pubmed/16434698
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnj011
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