Topologically Fixed SecG Is Fully Functional

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly...

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Bibliografiske detaljer
Main Authors: van der Sluis, Eli O., van der Vries, Erhard, Berrelkamp, Greetje, Nouwen, Nico, Driessen, Arnold J. M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2006
Fag:
Microbial Cell Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1347354/
https://ncbi.nlm.nih.gov/pubmed/16428426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.188.3.1188-1190.2006
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