Determination of affinity, stoichiometry and sequence selectivity of minor groove binder complexes with double-stranded oligodeoxynucleotides by electrospray ionization mass spectrometry

Electrospray mass spectrometry was evaluated regarding the reliability of the determination of the stoichiometries and equilibrium association constants from single spectra. Complexes between minor groove binders (Hoechst 33258, Hoechst 33342, DAPI, netropsin and berenil) and 12mer oligonucleotide d...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Rosu, Frederic, Gabelica, Valérie, Houssier, Claude, De Pauw, Edwin
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2002
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134255/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12177310/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf081
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