Tilted view reconstruction in optical microscopy. Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei.

The resolution along the optical axis (z) is much less than the in-plane resolution in any current optical microscope, conventional or otherwise. We have used mutually tilted, through-focal section views of the same object to provide a solution to this problem. A tilting specimen stage was construct...

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Main Authors: Shaw, P J, Agard, D A, Hiraoka, Y, Sedat, J W
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1330446/
https://ncbi.nlm.nih.gov/pubmed/2495031
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