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Reference phase analysis of free and bound intracellular solutes. I. Sodium and potassium in amphibian oocytes.

A method is described for the quantitative determination of free and bound solute concentrations in the cytoplasm of intact cells. The method includes (a) introduction of a gelatin gel reference phase (RP) into the cytoplasm; (b) diffusion of dissolved substances between cytoplasm and RP, (c) cell q...

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Autors principals: Horowitz, S B, Paine, P L, Tluczek, L, Reynhout, J K
Format: Artigo
Idioma:Inglês
Publicat: 1979
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1328446/
https://ncbi.nlm.nih.gov/pubmed/262385
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