Mutagenically Separated PCR Assay for Rapid Detection of M41L and K70R Zidovudine Resistance Mutations in CRF01_AE (Subtype E) Human Immunodeficiency Virus Type 1

A rapid zidovudine (ZDV) resistance genotypic assay was developed based on the mutagenically separated PCR (MS-PCR) technique to detect two ZDV-resistant mutations, M41L and K70R in CRF01_AE (subtype E). Endpoint dilution analysis revealed that the newly constructed MS-PCR assay could successfully d...

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Detalles Bibliográficos
Autores principales: Myint, Lay, Ariyoshi, Koya, Yan, Hua, Frater, Alexander J., Auwanit, Wattana, Pathipvanith, Panita, Yamada, Kaneo, Matsuda, Masakazu, Chiba, Tomoko, Fujita, Kazunori, McClure, Myra, Weber, Jonathan N., Sugiura, Wataru
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2002
Materias:
Antiviral Agents
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC132767/
https://ncbi.nlm.nih.gov/pubmed/12435689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.46.12.3861-3868.2002
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