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Flow cytometric detection of specific RNAs in native human cells with quenched autoligating FRET probes

We describe the use of modified fluorescent-labeled oligonucleotide probes in the sequence-specific detection of messenger RNAs in live human cells. To make this detection possible, we developed a previously undescribed probe design that combines earlier quenched autoligation chemistry with a previo...

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Bibliografski detalji
Glavni autori: Abe, Hiroshi, Kool, Eric T.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2006
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1326184/
https://ncbi.nlm.nih.gov/pubmed/16384914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0509938103
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