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A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame

BACKGROUND: Hepatitis C virus (HCV) is a pathogenic hepatic flavivirus with a single stranded RNA genome. It has a high genetic variability and is classified into six major genotypes. Genotype 1a and 1b cause the majority of infections in the USA. Viral genomic sequence information is needed to corr...

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Detalhes bibliográficos
Main Authors: Yao, Ermei, Tavis, John E
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1325262/
https://ncbi.nlm.nih.gov/pubmed/16321149
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-2-88
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