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Construction of Engineered Bifunctional Enzymes and Their Overproduction in Aspergillus niger for Improved Enzymatic Tools To Degrade Agricultural By-Products

Two chimeric enzymes, FLX and FLXLC, were designed and successfully overproduced in Aspergillus niger. FLX construct is composed of the sequences encoding the feruloyl esterase A (FAEA) fused to the endoxylanase B (XYNB) of A. niger. A C-terminal carbohydrate-binding module (CBM family 1) was grafte...

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Autori principali: Levasseur, Anthony, Navarro, David, Punt, Peter J., Belaïch, Jean-Pierre, Asther, Marcel, Record, Eric
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317392/
https://ncbi.nlm.nih.gov/pubmed/16332795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8132-8140.2005
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