Triphenylmethane Reductase from Citrobacter sp. Strain KCTC 18061P: Purification, Characterization, Gene Cloning, and Overexpression of a Functional Protein in Escherichia coli

We purified to homogeneity an enzyme from Citrobacter sp. strain KCTC 18061P capable of decolorizing triphenylmethane dyes. The native form of the enzyme was identified as a homodimer with a subunit molecular mass of about 31 kDa. It catalyzes the NADH-dependent reduction of triphenylmethane dyes, w...

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Detalhes bibliográficos
Main Authors: Jang, Moon-Sun, Lee, Young-Mi, Kim, Cheorl-Ho, Lee, Jai-Heon, Kang, Dong-Woo, Kim, Seok-Jo, Lee, Young-Choon
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317337/
https://ncbi.nlm.nih.gov/pubmed/16332773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.7955-7960.2005
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