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A Novel Real-Time PCR for Listeria monocytogenes That Monitors Analytical Performance via an Internal Amplification Control
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-...
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Autors principals: | , , , , |
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Format: | Artigo |
Idioma: | Inglês |
Publicat: |
American Society for Microbiology
2005
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Matèries: | |
Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1317324/ https://ncbi.nlm.nih.gov/pubmed/16332910 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.9008-9012.2005 |
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