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A Novel Real-Time PCR for Listeria monocytogenes That Monitors Analytical Performance via an Internal Amplification Control

We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-...

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Bibliographische Detailangaben
Hauptverfasser: Rodríguez-Lázaro, David, Pla, Maria, Scortti, Mariela, Monzó, Héctor J., Vázquez-Boland, José A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317324/
https://ncbi.nlm.nih.gov/pubmed/16332910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.9008-9012.2005
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