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The in situ observation of the temperature and pressure stability of recombinant Aspergillus aculeatus pectin methylesterase with Fourier transform IR spectroscopy reveals an unusual pressure stability of β-helices
The stability of recombinant Aspergillus aculeatus PME (pectin methylesterase), an enzyme with high β-helix content, was studied as a function of pressure and temperature. The conformational stability was monitored using FTIR (Fourier transform IR) spectroscopy whereas the functional enzyme stabilit...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Portland Press Ltd.
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1316296/ https://ncbi.nlm.nih.gov/pubmed/16050809 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20050721 |
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