Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus

BACKGROUND: The GP5+/GP6+ PCR assay is a well-established HPV detection technique. This study has examined the effects of incorporating 'hot start' and 'touchdown' steps into the protocol. In addition, dTTP was substituted with dUTP to permit contamination control measures agains...

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Detaylı Bibliyografya
Asıl Yazarlar: Evans, Mark F, Adamson, Christine SC, Simmons-Arnold, Linda, Cooper, Kumarasen
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: BioMed Central 2005
Konular:
Technical Advance
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1314887/
https://ncbi.nlm.nih.gov/pubmed/16288661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6890-5-10
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