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Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus

BACKGROUND: The GP5+/GP6+ PCR assay is a well-established HPV detection technique. This study has examined the effects of incorporating 'hot start' and 'touchdown' steps into the protocol. In addition, dTTP was substituted with dUTP to permit contamination control measures agains...

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Detalhes bibliográficos
Main Authors: Evans, Mark F, Adamson, Christine SC, Simmons-Arnold, Linda, Cooper, Kumarasen
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1314887/
https://ncbi.nlm.nih.gov/pubmed/16288661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6890-5-10
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