Human L1 element target-primed reverse transcription in vitro

L1 elements are ubiquitous human transposons that replicate via an RNA intermediate. We have reconstituted the initial stages of L1 element transposition in vitro. The reaction requires only the L1 ORF2 protein, L1 3′ RNA, a target DNA and appropriate buffer components. We detect branched molecules...

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Bibliografske podrobnosti

izdano v:	EMBO J
Principais autores:	Cost, Gregory J., Feng, Qinghua, Jacquier, Alain, Boeke, Jef D.
Format:	Artigo
Jezik:	Inglês
Izdano:	Nature Publishing Group 2002
Teme:	Articles
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC131089/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12411507/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/cdf592
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