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Cytochrome c conformations resolved by the photon counting histogram: Watching the alkaline transition with single-molecule sensitivity
We apply the photon counting histogram (PCH) model, a fluorescence technique with single-molecule sensitivity, to study pH-induced conformational changes of cytochrome c. PCH is able to distinguish different protein conformations based on the brightness of a fluorophore sensitive to its local enviro...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1308922/ https://ncbi.nlm.nih.gov/pubmed/16314563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0508975102 |
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