Rapid Typing of Bordetella pertussis Pertussis Toxin Gene Variants by LightCycler Real-Time PCR and Fluorescence Resonance Energy Transfer Hybridization Probe Melting Curve Analysis

A LightCycler real-time PCR hybridization probe assay was developed for rapid typing of gene variants of the Bordetella pertussis virulence factor pertussis toxin. The assay correctly identified the ptxS1 alleles of all strains tested, comprising 57 Finnish clinical isolates and 2 vaccine strains. T...

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Bibliographische Detailangaben
Hauptverfasser: Mäkinen, Johanna, Mertsola, Jussi, Viljanen, Matti K., Arvilommi, Heikki, He, Qiushui
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2002
Schlagworte:
Bacteriology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC130802/
https://ncbi.nlm.nih.gov/pubmed/12037089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.6.2213-2216.2002
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