Detection of Smallpox Virus DNA by LightCycler PCR

A 300-bp plasmid fragment of the hemagglutinin gene was used as target DNA to develop a rapid real-time LightCycler (Roche Applied Science, Indianapolis, Ind.) PCR assay for laboratory detection of smallpox virus. PCR primers and probes were designed specifically for detection of smallpox virus DNA,...

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Bibliographic Details
Main Authors: Espy, Mark J., Cockerill III, Franklin R., Meyer, Richard F., Bowen, Michael D., Poland, Gregory A., Hadfield, Ted L., Smith, Thomas F.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC130682/
https://ncbi.nlm.nih.gov/pubmed/12037052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.6.1985-1988.2002
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