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Single-Molecule Enzymology of Chymotrypsin Using Water-in-Oil Emulsion
Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1305659/ https://ncbi.nlm.nih.gov/pubmed/15764670 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.055053 |
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