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Mie-Type Scattering and Non-Beer-Lambert Absorption Behavior of Human Cells in Infrared Microspectroscopy

We report infrared microspectral features of nuclei in a completely inactive and contracted (pyknotic) state, and of nuclei of actively dividing cells. For pyknotic nuclei, the very high local concentration of DNA leads to opaqueness of the chromatin and, consequently, the absence of DNA signals in...

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Detalhes bibliográficos
Main Authors: Mohlenhoff, Brian, Romeo, Melissa, Diem, Max, Wood, Bayden R.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1305510/
https://ncbi.nlm.nih.gov/pubmed/15749767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.057950
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