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Sequence-specific DNA binding by the vnd/NK-2 homeodomain of Drosophila

The ventral nervous system defective (vnd)/NK-2 homeodomain and some flanking amino acid residues were expressed in Escherichia coli, purified to homogeneity, and the protein was covalently coupled to Sepharose. Oligodeoxynucleotides that contained 16-bp random sequences were purified by vnd/NK-2 af...

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Main Authors: Wang, Lan-Hsiang, Chmelik, Rebecca, Nirenberg, Marshall
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2002
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC130527/
https://ncbi.nlm.nih.gov/pubmed/12232052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.202461199
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