Binding of the Escherichia coli response regulator CheY to its target measured in vivo by fluorescence resonance energy transfer
In Escherichia coli chemotaxis, signaling depends on modulation of the level of phosphorylation of CheY, a small protein that couples receptors and flagellar motors. Working in vivo, we used fluorescence resonance energy transfer (FRET) to measure the interaction of CheY∼P with its target, FliM. Bin...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
2002
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC130518/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12232047/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.192463199 |
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