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Measuring Unfolding of Proteins in the Presence of Denaturant Using Fluorescence Correlation Spectroscopy
IFABP is a small (15 kDa) protein consisting mostly of antiparallel β-strands that surround a large cavity into which ligands bind. We have previously used FCS to show that the native protein, labeled with fluorescein, exhibits dynamic fluctuation with a relaxation time of 35 μs. Here we report the...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Biophysical Society
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1305143/ https://ncbi.nlm.nih.gov/pubmed/15556973 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.053199 |
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