Unfolding Studies on Soybean Agglutinin and Concanavalin A Tetramers: A Comparative Account

The unfolding pathway of two very similar tetrameric legume lectins soybean agglutinin (SBA) and Concanavalin A (ConA) were determined using GdnCl-induced denaturation. Both proteins displayed a reversible two-state unfolding mechanism. The analysis of isothermal denaturation data provided values fo...

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Bibliographic Details
Main Authors: Sinha, Sharmistha, Mitra, Nivedita, Kumar, Gyanendra, Bajaj, Kanika, Surolia, Avadhesha
Format: Artigo
Language:Inglês
Published: Biophysical Society 2005
Subjects:
Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1305132/
https://ncbi.nlm.nih.gov/pubmed/15542553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.051052
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