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Lipid Bilayer Topology of the Transmembrane α-Helix of M13 Major Coat Protein and Bilayer Polarity Profile by Site-Directed Fluorescence Spectroscopy

This article presents a new formalism to perform a quantitative fluorescence analysis using the Stokes shift of AEDANS-labeled cysteine mutants of M13 major coat protein incorporated in lipid bilayers. This site-directed fluorescence spectroscopy approach enables us to obtain the topology of the bil...

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Autors principals: Koehorst, Rob B. M., Spruijt, Ruud B., Vergeldt, Frank J., Hemminga, Marcus A.
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304553/
https://ncbi.nlm.nih.gov/pubmed/15345527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.043208
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