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High Sensitivity of Stark-Shift Voltage-Sensing Dyes by One- or Two-Photon Excitation Near the Red Spectral Edge

Sensitivity spectra of Stark-shift voltage sensitive dyes, such as ANNINE-6, suggest the use of the extreme red edges of the excitation spectrum to achieve large fractional fluorescence changes with membrane voltage. This was tested in cultured HEK293 cells. Cells were illuminated with light at the...

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Bibliografiske detaljer
Main Authors: Kuhn, Bernd, Fromherz, Peter, Denk, Winfried
Format: Artigo
Sprog:Inglês
Udgivet: Biophysical Society 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304385/
https://ncbi.nlm.nih.gov/pubmed/15240496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.040477
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