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Intrasequence GFP in Class I MHC Molecules, a Rigid Probe for Fluorescence Anisotropy Measurements of the Membrane Environment

Fluorescence anisotropy measurements can elucidate the microenvironment of a membrane protein in terms of its rotational diffusion, interactions, and proximity to other proteins. However, use of this approach requires a fluorescent probe that is rigidly attached to the protein of interest. Here we d...

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Detalhes bibliográficos
Main Authors: Rocheleau, Jonathan V., Edidin, Michael, Piston, David W.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302987/
https://ncbi.nlm.nih.gov/pubmed/12770911
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