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Transglutaminase-induced cross-linking between subdomain 2 of G-actin and the 636-642 lysine-rich loop of myosin subfragment 1.

G-actin was covalently cross-linked with S1 in a bacterial transglutaminase-catalyzed reaction. The cross-linking sites were identified with the help of fluorescent probes and limited proteolysis as the Gln-41 on the DNase I binding loop of subdomain 2 in G-actin and a lysine-rich loop (residues 636...

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Autores principales: Eligula, L, Chuang, L, Phillips, M L, Motoki, M, Seguro, K, Muhlrad, A
Formato: Artigo
Lenguaje:Inglês
Publicado: 1998
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302574/
https://ncbi.nlm.nih.gov/pubmed/9533706
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