Two-photon fluorescence lifetime imaging of the skin stratum corneum pH gradient.

Two-photon fluorescence lifetime imaging is used to identify microdomains (1-25 microm) of two distinct pH values within the uppermost layer of the epidermis (stratum corneum). The fluorophore used is 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF), whose lifetime tau (pH 4...

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Autors principals: Hanson, Kerry M, Behne, Martin J, Barry, Nicholas P, Mauro, Theodora M, Gratton, Enrico, Clegg, Robert M
Format: Artigo
Idioma:Inglês
Publicat: 2002
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302264/
https://ncbi.nlm.nih.gov/pubmed/12202391
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