A two-photon view of an enzyme at work: Crotalus atrox venom PLA2 interaction with single-lipid and mixed-lipid giant unilamellar vesicles.

We describe the interaction of Crotalus atrox-secreted phospholipase A2 (sPLA2) with giant unilamellar vesicles (GUVs) composed of single and binary phospholipid mixtures visualized through two-photon excitation fluorescent microscopy. The GUV lipid compositions that we examined included 1-palmitoyl...

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Bibliographische Detailangaben
Hauptverfasser: Sanchez, Susana A, Bagatolli, Luis A, Gratton, Enrico, Hazlett, Theodore L
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2002
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302016/
https://ncbi.nlm.nih.gov/pubmed/11916878
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