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Reconstitution of membrane proteins into giant unilamellar vesicles via peptide-induced fusion.

In this work, we present a protocol to reconstitute membrane proteins into giant unilamellar vesicles (GUV) via peptide-induced fusion. In principle, GUV provide a well-defined lipid matrix, resembling a close-to-native state for biophysical studies, including optical microspectroscopy, of transmemb...

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Main Authors: Kahya, N, Pécheur, E I, de Boeij, W P, Wiersma, D A, Hoekstra, D
格式: Artigo
語言:Inglês
出版: 2001
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301625/
https://ncbi.nlm.nih.gov/pubmed/11509360
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