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Highly nonlinear photodamage in two-photon fluorescence microscopy.

Two-photon fluorescence excitation is being increasingly used in laser scan microscopy due to very low photodamage induced by this technique under normal operation. However, excitation intensity has to be kept low, because nonlinear photodamage sets in when laser power is increased above a certain t...

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Bibliographic Details
Main Authors: Hopt, A, Neher, E
Format: Artigo
Language:Inglês
Published: 2001
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301392/
https://ncbi.nlm.nih.gov/pubmed/11259316
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