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Chain-selective isotopic labeling for NMR studies of large multimeric proteins: application to hemoglobin.

Multidimensional, multinuclear NMR has the potential to elucidate the mechanisms of allostery and cooperativity in multimeric proteins under near-physiological conditions. However, NMR studies of proteins made up of non-equivalent subunits face the problem of severe resonance overlap, which can prev...

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Autors principals: Simplaceanu, V, Lukin, J A, Fang, T Y, Zou, M, Ho, N T, Ho, C
Format: Artigo
Idioma:Inglês
Publicat: 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301010/
https://ncbi.nlm.nih.gov/pubmed/10920044
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