Direct observation of one-dimensional diffusion and transcription by Escherichia coli RNA polymerase.

The dynamics of nonspecific and specific Escherichia coli RNA polymerase (RNAP)-DNA complexes have been directly observed using scanning force microscopy operating in buffer. To this end, imaging conditions had to be found in which DNA molecules were adsorbed onto mica strongly enough to be imaged,...

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Main Authors: Guthold, M, Zhu, X, Rivetti, C, Yang, G, Thomson, N H, Kasas, S, Hansma, H G, Smith, B, Hansma, P K, Bustamante, C
Formato: Artigo
Idioma:Inglês
Publicado: 1999
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1300507/
https://ncbi.nlm.nih.gov/pubmed/10512846
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