Rapid Isolation of a Single-Chain Antibody against the Cyanobacterial Toxin Microcystin-LR by Phage Display and Its Use in the Immunoaffinity Concentration of Microcystins from Water

A naïve (unimmunized) human semisynthetic phage display library was employed to isolate recombinant antibody fragments against the cyanobacterial hepatotoxin microcystin-LR. Selected antibody scFv genes were cloned into a soluble expression vector and expressed in Escherichia coli for characterizati...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: McElhiney, Jacqui, Drever, Mathew, Lawton, Linda A., Porter, Andy J.
التنسيق: Artigo
اللغة:Inglês
منشور في: American Society for Microbiology 2002
الموضوعات:
Methods
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC129936/
https://ncbi.nlm.nih.gov/pubmed/12406716
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.11.5288-5295.2002
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