Improved System for Protein Engineering of the Hydroxylase Component of Soluble Methane Monooxygenase

Soluble methane monooxygenase (sMMO) of Methylosinus trichosporium OB3b is a three-component oxygenase that catalyses the O(2)- and NAD(P)H-dependent oxygenation of methane and numerous other substrates. Despite substantial interest in the use of genetic techniques to study the mechanism of sMMO and...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Smith, Thomas J., Slade, Susan E., Burton, Nicolas P., Murrell, J. Colin, Dalton, Howard
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2002
Subjects:	Enzymology and Protein Engineering
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129910/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406713/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5265-5273.2002
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