Fluorescence Resonance Energy Transfer Microscopy of the Helicobacter pylori Vacuolating Cytotoxin within Mammalian Cells

The Helicobacter pylori vacuolating cytotoxin (VacA) binds and enters mammalian cells to induce cellular vacuolation. To investigate the quaternary structure of VacA within the intracellular environment where toxin cytotoxicity is elaborated, we employed fluorescence resonance energy transfer (FRET)...

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Detalhes bibliográficos
Main Authors: Willhite, David C., Ye, Dan, Blanke, Steven R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Molecular Pathogenesis
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC128058/
https://ncbi.nlm.nih.gov/pubmed/12065526
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.70.7.3824-3832.2002
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