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Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels.

We have modified the procedure of Criado and Keller (1987) to study ion channels of Escherichia coli reconstituted in liposomes. The modifications include (a) excluding the use of any detergent and (b) inducing blisters from liposomes with Mg2+. These blisters, which appear to be unilamellar, are st...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Delcour, A H, Martinac, B, Adler, J, Kung, C
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1280516/
https://ncbi.nlm.nih.gov/pubmed/2477074
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