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Mutational Analysis of Ganglioside GM(1)-Binding Ability, Pentamer Formation, and Epitopes of Cholera Toxin B (CTB) Subunits and CTB/Heat-Labile Enterotoxin B Subunit Chimeras

Variants of cholera toxin B subunit (CTB) were made by bisulfite- and oligonucleotide-directed mutagenesis of the ctxB gene. Variants were screened by a radial passive immune hemolysis assay (RPIHA) for loss of binding to sheep erythrocytes (SRBC). Variant CTBs were characterized for the formation o...

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Autors principals: Jobling, Michael G., Holmes, Randall K.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC127768/
https://ncbi.nlm.nih.gov/pubmed/11854209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.70.3.1260-1271.2002
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