Rat liver betaine–homocysteine S-methyltransferase equilibrium unfolding: insights into intermediate structure through tryptophan substitutions

Equilibrium folding of rat liver BHMT (betaine–homocysteine methyltransferase), a TIM (triosephosphate isomerase)-barrel tetrameric protein, has been studied using urea as denaturant. A combination of activity measurements, tryptophan fluorescence, CD and sedimentation-velocity studies suggested a m...

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Bibliografske podrobnosti
Main Authors: Garrido, Francisco, Gasset, María, Sanz-Aparicio, Juliana, Alfonso, Carlos, Pajares, María A.
Format: Artigo
Jezik:Inglês
Izdano: Portland Press Ltd. 2005
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1276960/
https://ncbi.nlm.nih.gov/pubmed/15943585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20050505
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