Plasmidic versus Insertional Cloning of Heterologous Genes in Mycobacterium bovis BCG: Impact on In Vivo Antigen Persistence and Immune Responses

Bivalent recombinant strains of Mycobacterium bovis BCG (rBCG) expressing the early regulatory nef and the structural gag(p26) genes from the simian immunodeficiency virus (SIV) SIVmac251 were engineered so that both genes were cotranscribed from a synthetic operon. The expression cassette was clone...

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Detalhes bibliográficos
Main Authors: Méderlé, I., Bourguin, I., Ensergueix, D., Badell, E., Moniz-Peireira, J., Gicquel, B., Winter, N.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Microbial Immunity and Vaccines
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC127622/
https://ncbi.nlm.nih.gov/pubmed/11748196
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.70.1.303-314.2002
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