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Combining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome

Cloning the whole 3.5-megabase (Mb) genome of the photosynthetic bacterium Synechocystis PCC6803 into the 4.2-Mb genome of the mesophilic bacterium Bacillus subtilis 168 resulted in a 7.7-Mb composite genome. We succeeded in such unprecedented large-size cloning by progressively assembling and editi...

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Bibliografiske detaljer
Main Authors: Itaya, Mitsuhiro, Tsuge, Kenji, Koizumi, Maki, Fujita, Kyoko
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2005
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1276048/
https://ncbi.nlm.nih.gov/pubmed/16236728
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0503868102
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