Denaturation of Either Manduca sexta Aminopeptidase N or Bacillus thuringiensis Cry1A Toxins Exposes Binding Epitopes Hidden under Nondenaturing Conditions

The effect of polypeptide denaturation of Bacillus thuringiensis Cry1A toxins or purified Manduca sexta 120-kDa aminopeptidase N on the specificities of their interactions was investigated. Ligand and dot blotting experiments were conducted with (125)I-labeled Cry1Ac, Cry1Ac mutant (509)QNR-AAA(511)...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Daniel, Anu, Sangadala, Sreedhara, Dean, Donald H., Adang, Michael J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Invertebrate Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC127554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11976078/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.5.2106-2112.2002
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