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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis

1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris–sodium acetate–EDTA buffer for 0·5 to 3hr. Gels were scanned at 280 or 265mμ. Techniques are described for slicing and radioactive counting. 2. The mobility of RNA was inversely related to the sedimen...

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Bibliographic Details
Main Author: Loening, U. E.
Format: Artigo
Language:Inglês
Published: 1967
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1270235/
https://ncbi.nlm.nih.gov/pubmed/5339944
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