Influence of the Transposition of the Thermostabilizing Domain of Clostridium thermocellum Xylanase (XynX) on Xylan Binding and Thermostabilization

A xylanase gene, xynX, of Clostridium thermocellum had one thermostabilizing domain (TSD) between the signal peptide sequence and the catalytic domain (CD). The TSD of a truncated xylanase gene, xynX′(TSD-CD), was transpositioned from the N terminus to the C terminus of the CD by overlapping PCRs, a...

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Bibliographische Detailangaben
Hauptverfasser: Shin, Eun-Sun, Yang, Mi-Jeong, Hwa Jung, Kyung, Kwon, Eun-Ju, Jung, Jae Sung, Park, Seur Kee, Kim, Jungho, Yun, Han Dae, Kim, Hoon
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2002
Schlagworte:
Enzymology and Protein Engineering
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC126775/
https://ncbi.nlm.nih.gov/pubmed/12089033
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.7.3496-3501.2002
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