Efficient Insertion Mutagenesis Strategy for Bacterial Genomes Involving Electroporation of In Vitro-Assembled DNA Transposition Complexes of Bacteriophage Mu

An efficient insertion mutagenesis strategy for bacterial genomes based on the phage Mu DNA transposition reaction was developed. Incubation of MuA transposase protein with artificial mini-Mu transposon DNA in the absence of divalent cations in vitro resulted in stable but inactive Mu DNA transposit...

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Detalhes bibliográficos
Main Authors: Lamberg, Arja, Nieminen, Sari, Qiao, Mingqiang, Savilahti, Harri
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC126711/
https://ncbi.nlm.nih.gov/pubmed/11823210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.2.705-712.2002
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