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Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells

Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing capacities, RNA interference with synthetic siRNA is inefficient and cost intensive, especially for functi...

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Foilsithe in:Proc Natl Acad Sci U S A
Príomhchruthaitheoirí: Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., Bishop, J. Michael
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: National Academy of Sciences 2002
Ábhair:
Rochtain ar líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126604/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12096193/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.152327299
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