Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with Various Plasmids

We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 10(4) transformants per μg of DNA. Using this procedure, we identified several...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Serror, Pascale, Sasaki, Takashi, Ehrlich, S. Dusko, Maguin, Emmanuelle
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Genetics and Molecular Biology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126594/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11772607/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.1.46-52.2002
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