Cloning, Sequence Analysis, and Expression in Escherichia coli of the Gene Encoding an α-Amino Acid Ester Hydrolase from Acetobacter turbidans

The α-amino acid ester hydrolase from Acetobacter turbidans ATCC 9325 is capable of hydrolyzing and synthesizing β-lactam antibiotics, such as cephalexin and ampicillin. N-terminal amino acid sequencing of the purified α-amino acid ester hydrolase allowed cloning and genetic characterization of the...

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Detalhes bibliográficos
Main Authors: Polderman-Tijmes, Jolanda J., Jekel, Peter A., de Vries, Erik J., van Merode, Annet E. J., Floris, René, van der Laan, Jan-Metske, Sonke, Theo, Janssen, Dick B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC126590/
https://ncbi.nlm.nih.gov/pubmed/11772629
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.1.211-218.2002
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