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A Plasmid Selection System in Lactococcus lactis and Its Use for Gene Expression in L. lactis and Human Kidney Fibroblasts

We report the development of a nonantibiotic and nonpathogenic host-plasmid selection system based on lactococcal genes and threonine complementation. We constructed an auxotrophic Lactococcus lactis MG1363Δthr strain which carries deletions in two genes encoding threonine biosynthetic enzymes. To a...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Glenting, Jacob, Madsen, Søren M., Vrang, Astrid, Fomsgaard, Anders, Israelsen, Hans
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2002
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC126435/
https://ncbi.nlm.nih.gov/pubmed/12324356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.10.5051-5056.2002
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